Comparison of ORAGENE® and Mouthwashed-Based Saliva Collection Methods for Genomic DNA Isolation

Ayesha A. Hassan

Abstract


In recent years, saliva has been used as a non-invasive method of obtaining genomic DNA. Two common collection methods include mouthwash and commercially produced saliva kits. Here, a novel comparison between these two collection methods, using Scope® mouthwash and the Oragene®-Discover kit (OGR-250) from DNA Genotek Inc., was conducted to analyze differences in the quantity and quality of the DNA isolated, and cost effectiveness. The Oragene® kit yielded greater quantity of DNA, while Scope® mouthwash was more cost effective. The difference in yield was attributed to the larger volume of saliva obtained from the Oragene® kit. Isolation from both collection methods resulted in similar DNA quality.    

Depuis quelques années, la salive est utilisée comme une méthode non-invasive pour obtenir de l’ADN génomique. Deux méthodes de collection communes sont par rince-bouche et par des trousses commerciales de collection de salive. Ici, une comparaison entre ces deux méthodes, utilisant la rince-bouche Scope et la trousse Oragene-Discover (OGR-250) de DNA Genotek Ink, a été conduite afin d’analyser les différences dans la quantité et la qualité d’ADN isolée ainsi que dans l’efficacité du coût.  La trousse Oragene a recueilli plus d’ADN, alors que Scope était moins cher. La différence en quantité est attribuée au plus grand volume de salive qui est obtenu grâce à l’Oragene. L’isolation par les deux méthodes résultait en une qualité similaire d’ADN.


Keywords


mouthwash; DNA; genomics; saliva; hygiene

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References


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DOI: https://doi.org/10.13034/jsst.v8i2.77

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